Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium.
Identifieur interne : 000567 ( Main/Exploration ); précédent : 000566; suivant : 000568Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium.
Auteurs : Xianghua Wen [République populaire de Chine] ; Yannan Jia ; Jiaxi LiSource :
- Journal of hazardous materials [ 1873-3336 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Phanerochaete.
- métabolisme : Antibactériens, Oxytétracycline, Peroxidases, Tétracycline.
- Dépollution biologique de l'environnement, Peroxyde d'hydrogène.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Anti-Bacterial Agents, Oxytetracycline, Peroxidases, Tetracycline.
- enzymology : Phanerochaete.
- Biodegradation, Environmental, Hydrogen Peroxide.
Abstract
Pharmaceuticals have been attracting increasing attention in recent years as emerging contaminants, of which the most frequently detected kind in various environments are antibiotics. In this study, crude manganese peroxidase (MnP) prepared from the Phanerochaete chrysosporium, a white rot fungi, was taken as a highly efficient biocatalyst to degrade tetracycline (TC) and oxytetracycline (OTC) which are widely used antibiotics. The results show that 72.5% of 50mg/L of TC was degraded when added 40 U/L of MnP, while 84.3% of 50mg/L of OTC was degraded with the same amount of the catalyst added, both within 4h. The degradation rate was dependant on the pH and the temperature of the reaction system, and was likely sensitive to the concentration of H(2)O(2). With the pH at 2.96-4.80, the temperature at 37-40 degrees C, the Mn(2+) concentration higher than 0.1mM and up to 0.4mM, the H(2)O(2) concentration of 0.2mM, and the enzyme-substrate ratio above 2.0 U/mg, the degradation rate reached the highest. In addition, a separate series of experiments also show that the compensation of H(2)O(2) during the reaction process could improve the degradation of TC by MnP.
DOI: 10.1016/j.jhazmat.2010.01.005
PubMed: 20117880
Affiliations:
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In this study, crude manganese peroxidase (MnP) prepared from the Phanerochaete chrysosporium, a white rot fungi, was taken as a highly efficient biocatalyst to degrade tetracycline (TC) and oxytetracycline (OTC) which are widely used antibiotics. The results show that 72.5% of 50mg/L of TC was degraded when added 40 U/L of MnP, while 84.3% of 50mg/L of OTC was degraded with the same amount of the catalyst added, both within 4h. The degradation rate was dependant on the pH and the temperature of the reaction system, and was likely sensitive to the concentration of H(2)O(2). With the pH at 2.96-4.80, the temperature at 37-40 degrees C, the Mn(2+) concentration higher than 0.1mM and up to 0.4mM, the H(2)O(2) concentration of 0.2mM, and the enzyme-substrate ratio above 2.0 U/mg, the degradation rate reached the highest. In addition, a separate series of experiments also show that the compensation of H(2)O(2) during the reaction process could improve the degradation of TC by MnP.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20117880</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3336</ISSN><JournalIssue CitedMedium="Internet"><Volume>177</Volume><Issue>1-3</Issue><PubDate><Year>2010</Year><Month>May</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of hazardous materials</Title><ISOAbbreviation>J Hazard Mater</ISOAbbreviation></Journal><ArticleTitle>Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>924-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jhazmat.2010.01.005</ELocationID><Abstract><AbstractText>Pharmaceuticals have been attracting increasing attention in recent years as emerging contaminants, of which the most frequently detected kind in various environments are antibiotics. In this study, crude manganese peroxidase (MnP) prepared from the Phanerochaete chrysosporium, a white rot fungi, was taken as a highly efficient biocatalyst to degrade tetracycline (TC) and oxytetracycline (OTC) which are widely used antibiotics. The results show that 72.5% of 50mg/L of TC was degraded when added 40 U/L of MnP, while 84.3% of 50mg/L of OTC was degraded with the same amount of the catalyst added, both within 4h. The degradation rate was dependant on the pH and the temperature of the reaction system, and was likely sensitive to the concentration of H(2)O(2). With the pH at 2.96-4.80, the temperature at 37-40 degrees C, the Mn(2+) concentration higher than 0.1mM and up to 0.4mM, the H(2)O(2) concentration of 0.2mM, and the enzyme-substrate ratio above 2.0 U/mg, the degradation rate reached the highest. In addition, a separate series of experiments also show that the compensation of H(2)O(2) during the reaction process could improve the degradation of TC by MnP.</AbstractText><CopyrightInformation>Copyright (c) 2010 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Xianghua</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>The State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. xhwen@tsinghua.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Yannan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jiaxi</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>01</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Hazard Mater</MedlineTA><NlmUniqueID>9422688</NlmUniqueID><ISSNLinking>0304-3894</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>F8VB5M810T</RegistryNumber><NameOfSubstance UI="D013752">Tetracycline</NameOfSubstance></Chemical><Chemical><RegistryNumber>X20I9EN955</RegistryNumber><NameOfSubstance UI="D010118">Oxytetracycline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="Y">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010118" MajorTopicYN="N">Oxytetracycline</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013752" MajorTopicYN="N">Tetracycline</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>08</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>12</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>01</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20117880</ArticleId><ArticleId IdType="pii">S0304-3894(10)00021-X</ArticleId><ArticleId IdType="doi">10.1016/j.jhazmat.2010.01.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Jia, Yannan" sort="Jia, Yannan" uniqKey="Jia Y" first="Yannan" last="Jia">Yannan Jia</name><name sortKey="Li, Jiaxi" sort="Li, Jiaxi" uniqKey="Li J" first="Jiaxi" last="Li">Jiaxi Li</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wen, Xianghua" sort="Wen, Xianghua" uniqKey="Wen X" first="Xianghua" last="Wen">Xianghua Wen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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